Genetische diversiteit

Dit artikel moet nog vertaald worden naar het Nederlands
 
Genetic diversity of the Maine Coon breed

 

Since a few years Optimal Selection has brought out a new complete DNA test. Next to the regular testing on HCM1, SMA and PK-def, this test provides something very cool: genetic diversity! A part of their database is set public by the owners of the cats. With the public profiles of Maine Coons, I was able to do a bit of research to give more insight on this new test genetic diversity % and how it relates to our conventional indicators.

This chart below shows the genetic diversity of the Maine Coon breed, which is between 28-42%. The average genetic diversity of the breed is at 34,5% at this point (22-1-2020). When more Maine Coons are being tested and added to this database in the future, the average and bandwidth could change. The chart is based on population A* and you can see that there are very few Maine Coons that scores in the outer areas. 

GenDiv.PNG
Does the Maine Coon breed have a healthy genetic diversity?

 

The Maine Coon is a natural breed, so you would expect it to be diverse. Although there are not many new Maine Coons added to the breed today, the breed is still considered to be diverse. But it depends on the lines that you breed with. Some are less diverse than others, that’s why testing is a good thing. With the breedertool you can find specific cats that comes from unrelated lines, that can improve the genetic diversity for your future offspring. If you have a less diverse cat now, you can easily improve it by choosing the right partner for future matings. The more cats are added and set on public, the more interesting this tool will get. It can help a breeder to improve the genetic diversity as a part of your breeding plan, besides the conventional indicators such as COI% (total inbreeding coefficient) and Clones%. It adds a new interesting perspective of information that breeders didn’t have before.

 

Compare different cat breeds

When you look at the Maine Coon compared to other cat breeds, you can see it is pretty high ranked on nr 7. As expected, the natural breeds are more genetically diverse than the breeds that were created by breeders. Note that this only shows the median (average). Every breed has it’s own bandwidth, some really small and some very wide. You can view the charts on mycatdna, by typing the breed you want to view, and click on the genetic diversity tab of a cat within that breed.

In the Netherlands, where I live, a common thought is that a mixed breed or a domestic cat (which in our case is a European shorthair) have a better health than a pedigreed cat because of their higher genetic diversity. Well the median of an European shorthair is indeed a bit higher than the median of a Maine Coon. The bandwidth of an European shorthair is between 35-42% and for a Maine Coon between 28-42%. BUT depending on the breeders goals and the lines the breeder is working with, it is definitely possible to breed Maine Coons that have at least as much genetic diversity as a mixed breed or domestic cat. Maine Coons that have a genetic diversity higher than 35% have reached that point. Obviously a breeder working with pedigreed cats has more knowledge of the lines and it’s health than a breeder who’s cat accidentally got pregnant by some neighbor’s cat we don’t know anything about. Which makes the choice for a Maine Coon even safer than a European shorthair where no history of the ancestors or are known and no healthtests are done.

The art of a good breeder is to create a healthy genetic diverse line without losing the characteristics that defines the breed (breeding standard). Considering reality is much more complex when you have to think about temper, hereditary diseases, fertility issues, befriended breeders around, showresults and many other factors that can have an impact on your breeding program.

Median per breed.PNG
How can you use genetic diversity in your breeding program?

 

When I learned about this test, my first question was: how does the genetic diversity relate to inbreeding levels? You would expect to see a higher genetic diversity of cats who have low inbreeding levels. But is this true for every cat? I had to find out by doing research on the public Maine Coon profiles in mycatdna.com. I looked up the pedigree names in the Pawpedsdatabase in order to find information about the inbreeding levels and clones. Then I have made the charts to visualize the relationship between the indicators that we use: total COI%, inbreeding on 5 generations, inbreeding on 10 generations and the clones. Below are the results for each indicator and the relationship with the genetic diversity. All those charts are based on Population B*.

 

Genetic diversity related to COI%

 

Let’s look how the genetic diversity relates to the total inbreeding coefficient (COI). You can see the green line, showing the average COI for each genetic diversity %. For example the Maine Coons that have a genetic diversity of 39% (rounded) has an average COI of 5%. And a Maine Coon with a genetic diversity of 31% has an average COI of 19%. The yellow and blue lines show the bandwidth where the COI% can be on an individual base. It shows that there is at least one Maine Coon that has a high genetic diversity of 38% and also has a high COI of 15%. It also shows that there was at least one Maine Coon that has a low genetic diversity of 30% and has a COI of 0%. This shows that probably not all New Foundation cats (COI 0%) are genetically diverse (but this test can prove if they are!). The black dotted line shows how many Maine Coons fall into the genetic diversity category. The outer edges don’t have as much testresults to make good conclusions here. But the trend (green line) is very visible. In other words, there is a relationship visible between the COI and the genetic diversity, but it is not as predictable since there are exceptions. A Maine coon with COI of 15% is likely to have a genetic diversity less than 35%, but you can see from the blue line that 38% is still possible also. The Maine Coons that have lower inbreeding levels then 10% will probably have a genetic variation level between 36,5 and 40,5%. In a low COI% pedigree nowadays often one or more New Foundation cats are responsible for the low numbers. But the genetic diversity does depend on the genetic diversity level of the New Foundation cat. This adds new information about the genetic diversity of that new line and if it indeed adds something new to the breed.

GenDiv vs COI.PNG
Genetic diversity related to inbreeding and linebreeding

 

So we have looked to the total COI levels, but what about the inbreeding levels that are closer in the pedigree? I’ve added two categories here. The inbreeding level within 5 generations, that shows how related the cats are looking only at the last 5 generations. The second one is the linebreeding that was done in the last 10 generations, how many double ancestors do you find there? Although there are not many examples of extremely inbreeding in the database to derive good conclusions from it, you can still see from the chart below, that in order to be on the higher side of genetic variation, it is wise to keep the inbreeding levels of the last 10 generations of your future matings below 2% (results > 34%) or preferable below 1% (results > 37%). That being said, a Maine Coon with 10 generation inbreeding level of 6% can still have a genetic diversity of 38%. It is a small chance, but it happens. I guess it was the lucky cat that inherited the unrelated parts of the related parents.

 

In case you didn’t already realize that inbreeding is not a good idea healthwise, the breeder tool helps you visualizing the results when you plan to mate related cats.

 

Example 1:

Brother – sister mating increases the COI with 25%. What happens with the genetic diversity when you plan such a mating (which is not recommended)? Take the profile of Chelles of Macadamia (genetic diversity of 39,2%) and select her brother Lyon of Macadamia (genetic diversity of 37,8%) in the breeder tool (select Sweden and scroll down to the last page to see Lyon). Note that brother and sister have a difference of 1,4%, which shows that not all siblings have the same diversity. Their kittens are expected to have a genetic diversity around 29,7%. A decrease of 8,8% in genetic diversity. You don’t get them much lower than that! Obviously a very bad idea.

 

Example 2:

Grandparent – grandchild mating increases the COI with 12,5%. We take the profile of Chelles of Macadamia (39,2%) again and this time we will look up her grandfather So Out Coon’s Mirai (38,5%). Both cats with a very good genetic diversity. The expected genetic variation of their kittens will be around 34,9%. A decrease of nearly 3,95% in genetic variation in just one generation. It seems a small number and small step, but don't take a few percent very lightly. It takes you straight to the other side of the chart. Remember the bandwidth of the Maine Coon is only 14% (between 28-42%), so a decrease of 3,95% means you will lose 28% of their genetic variation in one step.

Example 3:

Halfbrother and halfsister mating increases the COI with 12,5%. We look at the profile of Lyon of Macadamia (genetic diversity of 37,8%) in the breeder tool and we search for his halfsister FelineBloss Rose van Ashamta (39,1%). Together their kittens expect to have a genetic variation of 33,6%. A loss of 4,85% in genetic variation.

Note that mycatdna database doesn't keep track of any family relationships. The results is based purely by comparing the DNA. You can tell by matching two cats if their kittens decrease it means their DNA is mostly the same (and they are probably related).

GenDiv vs Inbreeding.PNG

Dit is een paragraaf. Klik hier om je eigen tekst toe te voegen.

GenRelations.PNG
Genetic diversity related to the Clones

 

How is the genetic diversity related to the clones? We know that a higher clone% means you have a lot of the same ancestors. When you don't know about the clones, please read more about my article "The influence of the clones" first.

When you are breeding outcross cats by keeping the clones below 20%, the cats will most likely all have a genetic diversity higher than 36%. On average they are even higher than 38%. Which is very good news and proof for every outcross breeder, that you are indeed working on a higher genetic diversity with outcross lines. Of course you already knew that, but it is good to have some prove!

Knowing that the average clone percentage is around 35%, this chart shows that the majority of these cats are on the left side of the chart, having a genetic diversity below 35%.

So I think we can say that there is a relationship between the genetic diversity and the clones. What fascinates me is the question why these lines are more rounded? The previous chart of the COI showed a more straight line.

GenDiv vs clones.PNG
Genetic diversity per country

 

This is a fun chart, but it is also the least reliable chart of all. Since some of the countries only have very few cats tested you can’t really jump into conclusions yet. The country where the cat was born (and where the breeder is located) is the country where the cat counts for. This chart shows only the countries which have a 3 cats minimum inside the public database. This chart is based on Population C.

The black line shows how many cats it contains and therefor you get a more reliable picture for the countries that have more cats tested. As expected, the Maine Coons with the highest variation are found in the US, as New Foundation cats all come from the US (or CA). But you can see that they also have quite a lot of cats with a lower genetic variation, which is most probably caused (but I am assuming here) by American breeders importing less diverse cats from overseas.

You can also see that Sweden is doing very well keeping the genetic variation high. Of all the countries on the left side, Russia sticks out with a lower genetic diversity of on average 33%. I have noticed that most of the Russian cats who are in the mycatdna database are now owned by American breeders, which will likely bring their genetic variation down within their breeding programs. If this assumption is correct it will be shown in future tested generations.

GenDiv per country.PNG
If you take out the countries that has less than 12 cats tested, you are left with the top 5 countries that have the most cats tested within mycatdna. This chart is based on Population D.
GenDiv per country top 5.PNG
Conclusion

 

The genetic diversity shows to be a promising new indicator. Since chance also plays a part, it is less predictable than the usual indicators as COI, Clones and Top5. It could be more challenging to integrate this into your breedingprogram. With low inbreeding levels you are aiming for higher diversity, but with this test you will actually KNOW it's genetics diversity. This test is more factual and therefor reliable than the other indicators, so it certainly has a lot of value. Of course we mustn’t stare blind on the diversity alone though, diversity doesn’t necessary means the cat has quality genes. You could still end up with a high genetically diverse cat who inherited the wrong genes that messes things up. Also the genetic information should never replace any HCM/PKD test by echo, HD/PL test or other clinical health checks carried out by a veterinarian or specialist. This test is only 1 part of the total healthtests that a breeder is recommended to do.

But I must say I am a fan of this test and very enthusiastic about the positive impact that the breeding tool can have in the future, when more cats are added. It could be fun (or scary, depending the direction of the trend) to follow the trends for the upcoming years.

 

Population and source:

On the website of mycatdna, I’ve took the whole population of Maine Coons (polydactyl included) from the public cats. There are more Maine Coons tested but their profile can’t be seen by me, so I could only work with the public cats. On January 22th, 2020 the population of public Maine Coons were 288 testresults. From those cats, I have looked up their pedigree into the Pawpeds database to search for information about COI, inbreeding of 5, 10 generations and the clones. I could find 212 of the cats in the Pawpedsdatabase. From those 212 cats I also wrote down which country they are born. For the 76 cats that were not found in Pawpeds, I took the country information from the mycatdna profile.

 

*Used populations shown in the charts above:

  • Population A: 288 Maine Coons
    Total public Maine Coon profiles on mycatdna.com at 22-01-2020 (polydactyl included)

  • Population B: 212 Maine Coons
    Population A minus the 76 cats that could not be found in the Pawpeds database

  • Population C: 277 Maine Coons
    Population A minus the cats that were born in countries where only one or two cats are published

  • Population D: 221 Maine Coons
    Population A minus the cats that were born in countries where less than 12 cats are published

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